| Tumor | Detail |
|---|---|
| ESCC | Esophageal squamous cell Carcinoma |
| LUSC | Lung squamous cell carcinoma |
| GBM | Glioblastoma multiforme |
| HNSC | Head and Neck squamous cell carcinoma |
| PAAD | Pancreatic adenocarcinoma |
| LUAD | Lung adenocarcinoma |
| UCEC | Uterine Corpus Endometrial Carcinoma |
| KIRC | Kidney renal clear cell carcinoma |
| LIHC | Liver hepatocellular carcinoma |
| OV | Ovarian serous cystadenocarcinoma |
| STAD | Stomach adenocarcinoma |
| BRCA | Breast invasive carcinoma |
| COAD | Colon adenocarcinoma |
MaxQuant software (version 2.0.2.0) was used to analyze MS raw files. If the “internal reference”, such as a mixed sample, exists, the reference channel will be set according to the corresponding plex, and the normalization method will be set as ‘Weighted ratio to reference channel’. The expression ratios output by MaxQuant were log2-transformed and normalized using the median centering method across proteins and phosphorylation sites.
the meaning of ‘log2 (median)’ represented the log2 transformed median value of normalized expression of proteins or phosphorylation. log2 (median) (N) represented the log2 transformed median value in normal samples. log2 (median) (T) represented the log2 transformed median value in tumor samples.
The distribution of phosphorylation sites in specific protein is shown by lollipop plot.
X-axis represents amino acids of specific protein.
Y-axis represents the number of samples in which the phosphorylation site was identified.
The abundance of phosphorylation sites in specific protein is shown by heat maps.
X-axis respectively represents the median normalized abundance in non-tumor samples and in tumor samples.
Y-axis represents phosphorylation sites that can be quantified in specific tumor type.